Microelectrode Measurements on Red Beet Vacuole
نویسندگان
چکیده
منابع مشابه
Effect of sodium on potassium fluxes at the cell membrane and vacuole membrane of red beet.
Slices of red beet (Beta vulgaris) washed for 5 to 6 days are known to accumulate Na(+) in preference to K(+) from solutions containing both ions. The present work, using ion concentrations of 1.0 mm or less, with Ca(2+) added in some cases, shows that Na(+) strongly inhibits K(+) influx at the cell membrane (plasmalemma) while K(+) efflux is increased to a lesser extent. This result from compa...
متن کاملPurification of an h-translocating inorganic pyrophosphatase from vacuole membranes of red beet.
An H(+)-translocating inorganic pyrophosphatase (PPase) was isolated and purified from red beet (Beta vulgaris L.) tonoplast. One major polypeptide of molecular weight 67 kilodalton copurified with fluoride-inhibitable PPase activity when subjected to one-dimensional polyacrylamide gel electrophoresis. Overall, a 150-fold purification of the PPase was obtained, from the tonoplast fraction, thro...
متن کاملInvertase inhibitors from red beet, sugar beet, and sweet potato roots.
Invertase inhibitors have been isolated and partially purified from red beets, sugar beets, and sweet potatoes. These inhibitors are thermolabile proteins with molecular weights of 18,000 to 23,000. They do not inhibit yeast and Neurospora invertases, but they are reactive with potato tuber invertase and other plant invertases with pH optima near 4.5. There are differences in reactivity of the ...
متن کاملTarget Molecular Size of the Red Beet Plasma Membrane
Radiation inactivation of the red beet (Beta vulgaris L.) plasma membrane ATPase was carried out using -y-ray radiation from a '37Cs source. Inactivation of vanadate-sensitive ATPase activity by y-ray radiation followed an exponential decline with increasing total dose, indicating a single target size calculated to have a molecular weight of about 228,000. Since the catalytic subunit of the red...
متن کاملHow far can sodium substitute for potassium in red beet?
Sodium (Na) movement between plants and humans is one of the more critical aspects of bioregenerative systems of life support, which NASA is studying for the establishment of long-term bases on the Lunar or Martian surface. This study was conducted to determine the extent to which Na can replace potassium (K) in red beet (Beta vulgaris L. ssp vulgaris) without adversely affecting metabolic func...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1987
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.85.3.608